Finding Orders in Heterogeneity: Studying X Chromosome Inactivation using Single Cell Transcriptome
Dr. Kyoung-Jae Won
Department of Genetics, Institute for Diabetes Obesity and Metabolism 
University of Pennsylvania

Abstract: We studied X chromosome inactivation (XCI) during the human embryonic cell (hESC) differentiation using single-cell transcriptomic data. Cells were collected in hESCs and the differentiated cells by treating ESCs with BMP4 (Diffs). As expected, the expression levels of the master regulator (Oct4, Sox2, and Nanog) in ESCs were reduced drastically in Diffs. Interestingly, the expression level of Xist, a long intergenic non-coding RNA that controls XCI, was not correlated well with these master regulators. Classifying genes based on the Xist expression levels, we found a group of genes that are potentially regulated by Xist in ESCs. More importantly, we observed that many genes including the genes known to subject to XCI were not entirely inactivated in the inactivated X chromosome. Our analysis using single cell transcriptomic data expanded our knowledge about XCI.  In addition, I present a study about pancreatic cell development using single cell transcriptomic data. 

Tuesday, Feb. 16, 12:20 – 1:20 pm, in  the Science Lecture Hall

Date & Time
February 16, 2016
12:20 pm-1:20 pm

Event posted in Approved Campus Activity.